Artesunate: A natural product-based immunomodulator involved in human complement

• ART possesses anti-complement bioactivities. is involved in three effector pathways of complement interception. targets C1q likely via binding globular head region. specifically binds to the key therapeutic C1q, C3 and C5. A modified ELISA selectively allows activation. Complement an important innate immune defence machinery. Once dysregulated, it often linked pathogenesis diverse autoimmune diseases. Artesunate (ART) a well-known anti-malarial compound. Recently, has been highlighted by its potential effects on certain complement-related However, underlying mechanisms are hitherto unknown. In present study, we found that mediated interception as validated analysis haemolytic assay. cell-based setup using dying Jurkat cells, ART-mediated was also confirmed. Further, newly established system allowing activation classical pathway, lectin pathway alternative respectively. revealed dose-dependently inhibited C4 activation, terminal complex assembly pathways. blockade C5 with lesser extent properdin. The interaction determined be FACS ART-conjugated mesoporous silica particles bound microparticles. conclusion, for first time report bioactivities suggest benefit human